DNA

Part:BBa_K4621079:Design

Designed by: Ruikang Chen   Group: iGEM23_SCUT-China   (2023-10-05)


CRISPRi-62


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 4534
    Illegal PstI site found at 3
    Illegal PstI site found at 2428
    Illegal PstI site found at 2662
    Illegal PstI site found at 3874
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4511
    Illegal SpeI site found at 4534
    Illegal PstI site found at 3
    Illegal PstI site found at 2428
    Illegal PstI site found at 2662
    Illegal PstI site found at 3874
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 467
    Illegal BglII site found at 1262
    Illegal BglII site found at 4031
    Illegal BamHI site found at 207
    Illegal BamHI site found at 1556
    Illegal BamHI site found at 4499
    Illegal XhoI site found at 880
    Illegal XhoI site found at 3226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 4534
    Illegal PstI site found at 3
    Illegal PstI site found at 2428
    Illegal PstI site found at 2662
    Illegal PstI site found at 3874
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 4534
    Illegal PstI site found at 3
    Illegal PstI site found at 2428
    Illegal PstI site found at 2662
    Illegal PstI site found at 3874
    Illegal NgoMIV site found at 1824
    Illegal NgoMIV site found at 2195
    Illegal NgoMIV site found at 2398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3406
    Illegal BsaI.rc site found at 4285
    Illegal SapI site found at 798
    Illegal SapI site found at 2088
    Illegal SapI.rc site found at 3285


Design Notes

The effectiveness and GC content.


Source

This part is derived from PZ-ProP-Ect-i2 plasmid.

References