DNA
Part:BBa_K4621079:Design
Designed by: Ruikang Chen Group: iGEM23_SCUT-China (2023-10-05)
CRISPRi-62
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 15
Illegal SpeI site found at 4534
Illegal PstI site found at 3
Illegal PstI site found at 2428
Illegal PstI site found at 2662
Illegal PstI site found at 3874 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4511
Illegal SpeI site found at 4534
Illegal PstI site found at 3
Illegal PstI site found at 2428
Illegal PstI site found at 2662
Illegal PstI site found at 3874 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 467
Illegal BglII site found at 1262
Illegal BglII site found at 4031
Illegal BamHI site found at 207
Illegal BamHI site found at 1556
Illegal BamHI site found at 4499
Illegal XhoI site found at 880
Illegal XhoI site found at 3226 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 15
Illegal SpeI site found at 4534
Illegal PstI site found at 3
Illegal PstI site found at 2428
Illegal PstI site found at 2662
Illegal PstI site found at 3874 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 15
Illegal SpeI site found at 4534
Illegal PstI site found at 3
Illegal PstI site found at 2428
Illegal PstI site found at 2662
Illegal PstI site found at 3874
Illegal NgoMIV site found at 1824
Illegal NgoMIV site found at 2195
Illegal NgoMIV site found at 2398 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3406
Illegal BsaI.rc site found at 4285
Illegal SapI site found at 798
Illegal SapI site found at 2088
Illegal SapI.rc site found at 3285
Design Notes
The effectiveness and GC content.
Source
This part is derived from PZ-ProP-Ect-i2 plasmid.